LPS-binding systems and endothelial nitric oxide synthase in gout patients
https://doi.org/10.37489/2588-0519-GCP-0033
EDN: QALNGM
Abstract
Relevance. Gout is a common inflammatory arthropathy characterized by hyperuricemia, oxidative stress, and endothelial dysfunction.
Objective: To study the relationship between endotoxin-binding systems and the concentration of endothelial NO synthase (NOS3) in patients with gout.
Materials and methods. The study included 41 patients with a confirmed diagnosis of gout according to the EULAR/ACR classification criteria and 33 healthy people forming the control group. Plasma concentrations were measured: highly sensitive C-reactive protein (hsCRP) NOS3 (eNOS), BPI by solid-phase ELISA. Linear regression was used to assess the relationship between BPI and NOS3 levels and the Pearson correlation coefficient (r), coefficient of determination (R2), and signifi cance level (p) were calculated. IBM SPSS Statistics 26.0 soft ware was used.
Results. Patients with gout showed elevated levels of lipopolysaccharide-binding protein (LBP) and highly sensitive C-reactive protein, as well as significantly reduced BPI concentrations compared with the control group (p<0.001). Regression analysis showed a statistically significant positive correlation between BPI and NOS3 levels (r = 0.605, p<0.001), with 36.6 % of the NOS3 variance attributed to changes in BPI concentration.
Conclusion. The data obtained indicate a close relationship between innate immune systems and endothelial function in gout. BPI may have a protective effect that supports endothelial function in conditions of chronic inflammation and oxidative stress. In the future, it is possible to develop a comprehensive biomarker panel BPI+NOS3 for monitoring or predicting vascular complications and individualizing therapy for gout.
About the Authors
K. V Bubley
Vernadsky Crimean Federal University
Russian Federation
Competing Interests:
Russian Federation
Konstantin V. Bubley — Assistant Professor of the Department of Internal Medicine No. 2
Simferopol
Competing Interests:
The authors state that there is no conflict of interest
V. V. Beloglazov
Vernadsky Crimean Federal University
Russian Federation
Competing Interests:
Russian Federation
Vladimir A. Beloglazov — Dr. Sci. (Med.), professor, Head of the Department of Internal Medicine No. 2
Simferopol
Competing Interests:
The authors state that there is no conflict of interest
I. A. Yatskov
Vernadsky Crimean Federal University
Russian Federation
Competing Interests:
Russian Federation
Igor A. Yatskov — Cand. Sci. (Med.), associate professor, Department of Internal Medicine No. 2
Simferopol
Competing Interests:
The authors state that there is no conflict of interest
E. S. Ageeva
Vernadsky Crimean Federal University
Russian Federation
Competing Interests:
Russian Federation
Elizabeth S. Ageeva — Dr. Sci. (Med.), Head of the Department of Biology, Associate Professor of the Department of Biology
Simferopol
Competing Interests:
The authors state that there is no conflict of interest
E. V. Keledzhieva
The authors state that there is no conflict of interest
Russian Federation
Competing Interests:
Russian Federation
Emilia V. Keledzhieva — Cand. Sci. (Med.), Associate Professor of the Department of Medical Biology, Head of the Department of Medical Biology
Simferopol
Competing Interests:
The authors state that there is no conflict of interest
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Review
For citations:
Bubley K.V., Beloglazov V.V., Yatskov I.A., Ageeva E.S., Keledzhieva E.V. LPS-binding systems and endothelial nitric oxide synthase in gout patients. Kachestvennaya Klinicheskaya Praktika = Good Clinical Practice. 2026;(2):140-148. (In Russ.) https://doi.org/10.37489/2588-0519-GCP-0033. EDN: QALNGM
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