clinvestКачественная клиническая практикаKachestvennaya Klinicheskaya Praktika = Good Clinical Practice2588-05192618-8473ООО «Издательство ОКИ10.37489/2588-0519-GCP-0033QALNGMclinvest-870Research ArticleВНУТРЕННИЕ БОЛЕЗНИINTERNAL MEDICINEЛПС-связывающие системы и эндотелиальная синтаза оксида азота у больных подагройLPS-binding systems and endothelial nitric oxide synthase in gout patientshttps://orcid.org/0000-0002-8960-602XБублейК. В.BubleyK. V

Бублей Константин Викторович  — ассистент кафедры внутренней медицины № 2 2-го медицинского факультета

Симферополь

Konstantin V. Bubley  — Assistant Professor of the Department of Internal Medicine  No. 2

Simferopol

bubley.99@mail.ruhttps://orcid.org/0000-0001-9640-754XБелоглазовА. В.BeloglazovV. V.

Белоглазов Владимир Алексеевич — д. м. н., профессор, зав. кафедрой внутренней медицины № 2 2-го медицинского факультета

Симферополь

Vladimir A. Beloglazov — Dr. Sci. (Med.), professor, Head of the Department of Internal Medicine No. 2

Simferopol

biloglazov@mail.ruhttps://orcid.org/0000-0002-5486-7262ЯцковИ. А.YatskovI. A.

Яцков Игорь Анатольевич  — к. м. н., доцент кафедры внутренней медицины № 2 2-го медицинского факультета

Симферополь

Igor A. Yatskov — Cand. Sci. (Med.), associate professor, Department of Internal Medicine No. 2

Simferopol

egermd@yandex.ruhttps://orcid.org/0000-0003-4590-3580АгееваЕ. С.AgeevaE. S.

Агеева Елизавета Сергеевна — д. м. н., доцент кафедры медицинской биологии, зав. кафедрой медицинской биологии

Симферополь

Elizabeth S. Ageeva  — Dr. Sci. (Med.), Head of the Department of Biology, Associate Professor of the Department of Biology

Simferopol

ageevaeliz@rambler.ruhttps://orcid.org/0000-0002-1111-5079КеледжиеваЭ. В.KeledzhievaE. V.

Келеджиева Эмилия Владимировна — к. м. н., доцент кафедры медицинской биологии, зав. кафедрой медицинской биологии

Симферополь

Emilia V. Keledzhieva  — Cand. Sci. (Med.), Associate Professor of the Department of Medical Biology, Head of the Department of Medical Biology

Simferopol

emilia.keledzhieva@gmail.comФГАОУ ВО «Крымский федеральный университет им. В. И. Вернадского»РоссияVernadsky Crimean Federal UniversityRussian FederationФГАОУ ВО «Крымский федеральный университет им. В. И. Вернадского»РоссияThe authors state that there is no conflict of interestRussian Federation20263005202602140148Copyright © Бублей К.В., Белоглазов А.В., Яцков И.А., Агеева Е.С., Келеджиева Э.В., 20262026Бублей К.В., Белоглазов А.В., Яцков И.А., Агеева Е.С., Келеджиева Э.В.Bubley K.V., Beloglazov V.V., Yatskov I.A., Ageeva E.S., Keledzhieva E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.clinvest.ru/jour/article/view/870Актуальность

Актуальность. Подагра является распространённой воспалительной артропатией, характеризующейся гиперурикемией, окислительным стрессом и эндотелиальной дисфункцией.

Цель

Цель. Изучить взаимосвязь между эндотоксин-связывающими системами и  концентрацией эндотелиальной NO-синтазы (NOS3) у пациентов с подагрой. Материалы и методы. В исследование включены 41 пациент с подтверждённым диагнозом подагра согласно критериям классификации EULAR/ACR и 33 здоровых человека, формирующих контрольную группу. В плазме измеряли концентрации: вчСРБ (высокочувствительный С-реактивный белок), NOS3 (nitric oxide synthase 3; эндотелиальная синтаза оксида азота 3) и BPI (Bactericidal/Permeability-Increasing Protein) методом твердофазного иммуноферментного анализа (ИФА). Для оценки связи между уровнями BPI и NOS3 применяли линейную регрессию и рассчитали коэффициент корреляции Пирсона (r), коэффициент детерминации (R²) и уровень значимости (p). Использовалось программное обеспечение IBM SPSS Statistics 26.0.

Результаты

Результаты. У  пациентов с  подагрой выявлены повышенные уровни липополисахарид-связывающего белка (ЛСБ) и высокочувствительного С-реактивного белка, а также значительно сниженная концентрация BPI по сравнению с контрольной группой (p<0,001). Регрессионный анализ показал статистически значимую положительную корреляцию между уровнями BPI и NOS3 (r = 0,605, p<0,001), при этом 36,6 % дисперсии NOS3 объясняется изменениями концентрации BPI.

Выводы

Выводы. Полученные данные свидетельствуют о тесной взаимосвязи между системами врождённого иммунитета и эндотелиальной функцией при подагре. BPI может обладать протективным действием, поддерживающим функцию эндотелия в условиях хронического воспаления и окислительного стресса. В дальнейшем возможна разработка комплексной биомаркерной панели BPI+NOS3 для мониторинга или прогноза сосудистых осложнений и индивидуализации терапии при подагре.

Relevance

Relevance. Gout is a common inflammatory arthropathy characterized by hyperuricemia, oxidative stress, and endothelial dysfunction.

Objective

Objective: To study the relationship between endotoxin-binding systems and the concentration of endothelial NO synthase (NOS3) in patients with gout.

Materials and methods

Materials and methods. The study included 41 patients with a confirmed diagnosis of gout according to the EULAR/ACR classification criteria and 33 healthy people forming the control group. Plasma concentrations were measured: highly sensitive C-reactive protein (hsCRP) NOS3 (eNOS), BPI by solid-phase ELISA. Linear regression was used to assess the relationship between BPI and NOS3 levels and the Pearson correlation coefficient (r), coefficient of determination (R2), and signifi cance level (p) were calculated. IBM SPSS Statistics 26.0 soft ware was used.

Results

Results. Patients with gout showed elevated levels of lipopolysaccharide-binding protein (LBP) and highly sensitive C-reactive protein, as well as significantly reduced BPI concentrations compared with the control group (p<0.001). Regression analysis showed a statistically significant positive correlation between BPI and NOS3 levels (r = 0.605, p<0.001), with 36.6 % of the NOS3 variance attributed to changes in BPI concentration.

Conclusion

Conclusion. The data obtained indicate a close relationship between innate immune systems and endothelial function in gout. BPI may have a protective effect that supports endothelial function in conditions of chronic inflammation and oxidative stress. In the future, it is possible to develop a comprehensive biomarker panel BPI+NOS3 for monitoring or predicting vascular complications and individualizing therapy for gout.

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The authors declare that there are no conflicts of interest present.