Methylation of SHOX-2, DAPK1, RAR-beta, and Mir-375 genes in hydrothorax in patients with nephrotic syndrome
https://doi.org/10.37489/2588-0519-2024-3-68-72
EDN: ZKDXZM
Abstract
Nephrotic syndrome is characterized by clinical manifestations, including proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Hydrothorax is a rare but serious complication caused by enhanced fluid exudation due to hypoalbuminemia and changes in capillary permeability. In this study, the methylation of the SHOX-2, DAPK1, RAR-beta, and mir-37 genes was assessed in 35 patients with nephrotic syndrome and hydrothorax using DNA extracted from pleural fluid and urine. Methylation was determined using real-time PCR. The PCR results showed no methylation of SHOX-2, RAR-beta, DAPK1, or Mir-375. The amplification curves show an exponential increase in the signal and a stable plateau, indicating successful DNA amplification. The absence of methylation confirmed the high specificity of the method for detecting unmethylated sequences. These results highlight the need for further research to understand the epigenetic mechanisms of gene expression and to develop new therapeutic approaches aimed at modulating DNA methylation and restoring normal gene regulation.
About the Authors
V. E. Pokrovskiy
Federal Scientific and Clinical Center of the Federal Medical and Biological Agency
Russian Federation
Russian Federation
Vasiliy E. Pokrovskiy — postgraduate student Department of Internal Medicine
Moscow
A. N. Fedoseev
Federal Scientific and Clinical Center of the Federal Medical and Biological Agency
Russian Federation
Russian Federation
Anatoliy N. Fedoseev — Dr. Sci (Med.), Professor Department of Internal Medicine
Moscow
V. V. Smirnov
Federal Scientific and Clinical Center of the Federal Medical and Biological Agency
Russian Federation
Russian Federation
Vladimir V. Smirnov — Dr. Sci (Med.), Professor Head of the Department of Internal Medicine
Moscow
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Review
For citations:
Pokrovskiy V.E., Fedoseev A.N., Smirnov V.V. Methylation of SHOX-2, DAPK1, RAR-beta, and Mir-375 genes in hydrothorax in patients with nephrotic syndrome. Kachestvennaya Klinicheskaya Praktika = Good Clinical Practice. 2024;(3):68-72. (In Russ.) https://doi.org/10.37489/2588-0519-2024-3-68-72. EDN: ZKDXZM
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